LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2366..2462) /label=attL2 /note="attL2 (c)" misc_signal complement(2477..2496) /note="T7 Promoter/priming site (c)" primer_bind 2504..2520 /note="M13 Reverse priming site" gene 2633..3442 /note="Kanamycin resistance gene" rep_origin 3563..4236 /note="pUC origin" vector join(2374..4239,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2359 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:195841 IMAGE:100068224" /clone_lib="NIH_MGC_435" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 692..2359 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..2359 /gene="GALNT11" /db_xref="GeneID:63917" /db_xref="HGNC:19875" CDS 692..2359 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 11 (GalNAc-T11)" /gene="GALNT11" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 11 (GalNAc-T11)" /protein_id="AAI67834.1" /db_xref="GI:189442406" /db_xref="GeneID:63917" /db_xref="HGNC:19875" /translation="MGSVTVRYFCYGCLFTSATWTVLLFVYFNFSEVTQPLKNVPVKG SGPHGPSPKKFYPRFTRGPSRVLEPQFKANKIDDVIDSRVEDPEEGHLKFSSELGMIF NERDQELRDLGYQKHAFNMLISDRLGYHRDVPDTRNAACKEKFYPPDLPAASVVICFY NEAFSALLRTVHSVIDRTPAHLLHEIILVDDDSDFDDLKGELDEYVQKYLPGKIKVIR NTKREGLIRGRMIGAAHATGEVLVFLDSHCEVNVMWLQPLLAAIREDRHTVVCPVIDI ISADTLAYSSSPVVRGGFNWGLHFKWDLVPLSELGRAEGATAPIKSPTMAGGLFAMNR QYFHELGQYDSGMDIWGGENLEISFRIWMCGGKLFIIPCSRVGHIFRKRRPYGSPEGQ DTMTHNSLRLAHVWLDEYKEQYFSLRPDLKTKSYGNISERVELRKKLGCKSFKWYLDN VYPEMQISGSHAKPQQPIFVNRGPKRPKVLQRGRLYHLQTNKCLVAQGRPSQKGGLVV LKACDYSDPNQIWIYNEEHELVLNSLLCLDMSETRSSDPPRLMKCHGSGGSQQWTFGK NNRLYQVSVGQCLRAVDPLGQKGSVAMAICDGSSSQQWHLEG" misc_feature 692..2359 /note="GALNT11 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaaaaattc tatccccgtt tcactcgagg 721 cccaagtcga gtgctcgagc cacagttcaa agcaaacaaa attgacgatg tgatagacag 781 tcgtgttgaa gatccagaag aaggccactt gaaattctct tctgaattag gtatgatttt 841 taatgaacgc gatcaagagt tgagagactt gggctatcag aaacatgctt ttaatatgct 901 tatcagtgac cgcttgggct accacagaga tgtgccagac acaaggaatg cagcatgtaa 961 agaaaagttc tacccacctg acctgccagc tgctagtgtt gttatctgtt tctataatga 1021 agcgttttct gccttgcttc ggacagtgca cagtgtcata gaccgcacgc cagcacacct 1081 gcttcatgag atcatccttg tggatgatga tagtgacttt gatgatttga aaggagaact 1141 agatgaatat gtccaaaaat acctccctgg aaaaattaaa gtcataagaa atacaaagcg 1201 tgaggggttg attcgaggga gaatgattgg cgcggcccac gcgacaggag aagtccttgt 1261 gttcctggac agccactgtg aagtgaatgt gatgtggctg cagcccttgc tggccgccat 1321 ccgtgaggac cggcacaccg tggtgtgccc agtgattgac atcatcagcg ccgacacgct 1381 ggcctacagc tcgtcccctg tcgtccgcgg agggttcaac tggggactgc acttcaaatg 1441 ggatcttgtc cccctttctg agctaggacg agcggaggga gccactgcac caataaagtc 1501 accaacaatg gctggaggtt tgtttgccat gaacagacag tatttccatg aacttggaca 1561 gtatgatagt ggcatggata tctggggagg agaaaatttg gaaatatcat ttcggatctg 1621 gatgtgtggc ggtaagctct tcatcatccc ttgctctaga gtaggacaca ttttccgaaa 1681 aaggcgacca tatggatctc ccgaaggcca ggacaccatg acacacaact ctttgcggct 1741 ggcacatgtc tggttggatg aatacaagga gcagtatttt tccttaagac ctgacctgaa 1801 gacgaaaagc tatggcaata tcagtgagcg tgtggaactg agaaagaagt tgggctgtaa 1861 atcatttaaa tggtatttgg ataatgtata cccagagatg cagatatctg ggtcccacgc 1921 caaaccccaa caacccattt ttgtcaatag agggccaaaa cgacccaaag tccttcaacg 1981 tggaaggctc tatcacctcc agaccaacaa atgcctggtg gcccagggcc gcccaagtca 2041 gaagggaggt ctcgtggtgc ttaaggcctg tgactacagt gacccaaatc agatctggat 2101 ctataatgaa gagcatgaat tggttttaaa tagtctcctt tgtctagata tgtcagagac 2161 tcgctcatca gacccgccac ggctcatgaa atgccacggg tcaggaggat cccagcagtg 2221 gacctttggg aaaaacaatc ggctatacca ggtgtcggtt ggacagtgcc tgagagcagt 2281 ggatcccctg ggtcagaagg gctctgtcgc catggcgatc tgcgatggct cctcttcaca 2341 gcagtggcat ttggaaggtT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2401 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2461 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2521 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2581 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2641 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2701 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2761 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2821 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2881 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2941 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3001 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3061 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3121 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3181 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3241 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3301 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3361 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3421 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3481 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3541 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3601 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3661 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3721 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3781 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3841 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3901 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3961 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4021 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4081 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4141 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4201 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //